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... in the Yoshida effect. Escherichia coli cells,  pUC18 
Applied Microbiology and Biotechnology
Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect
Yoshida, N.; Ide, K.
, and chrysotile were used as recipient cells, ...
... by Shi et al. (2009a). The integrative vectors  pUC18 
World Journal of Microbiology and Biotechnology
Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis
Wang, Guanglu; Bai, Ling; Wang, Zhiwen; Shi, Ting; Chen, Tao; Zhao, Xueming
-TPA, ...
... provided by Prof. Gong Heng  K. pneumoniae/  pUC18 
Bioprocess and Biosystems Engineering
Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions
Zhao, Li; Zheng, Yu; Ma, Xingyuan; Wei, Dongzhi
K-dhaT Recombinant K. pneumoniae with plasmid ...
... μl) was performed with negatively supercoiled  pUC18 
BMC Molecular Biology
A functional type I topoisomerase from Pseudomonas aeruginosa
Jain, Teesta; Roper, Benjamin J; Grove, Anne
DNA (≈ 110 ng) unless otherwise stated, and the ...
... inactivation of glnA3; aprar This work  pUC18 
Archives of Microbiology
Investigation of the functional properties and regulation of three glutamine synthetase-like genes in Streptomyces coelicolor A3(2)
Rexer, H. U.; Schäberle, T.; Wohlleben, W.; Engels, A.
bla, lacZ’′α complementation system Vieira and ...
... or characteristics Source or reference Plasmids  pUC18 
Gut Pathogens
Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance
Feeney, Audrey; Johnston, Christopher D; Govender, Rodney; O’Mahony, Jim; Coffey, Aidan; Sleator, Roy D
Ampr, lacZ', pMB9 replicon [13] ...
... of trimers) and a DNA substrate: i) undigested  pUC18 
BMC Molecular Biology
Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Chen, Wen-yang; Ho, John WS; Huang, Jian-dong; Watt, Rory M
(184 ng); ii) PstI-linearized ...
... the parasite’s DNA, an external control (  pUC18 
Journal of Venomous Animals and Toxins including Tropical Diseases
Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
Gonçalves-de-Albuquerque, Suênia da Cunha; Pessoa e Silva, Rômulo; de Morais, Rayana Carla Silva; Trajano-Silva, Lays Adrianne Mendonça; Régis-da-Silva, Carlos Gustavo; Brandão-Filho, Sinval Pinto; de Paiva-Cavalcanti, Milena
) and an internal control (G3PD) for accurate ...
... Knabc We constructed an expression vector from  pUC18 
Saline Systems
NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?
Kurz, Matthias; Brünig, Anika; Galinski, Erwin
where the β-lactamase is replaced in-frame by ...
... the RNA. Materials and methods DNA and RNA The  pUC18 
Biotechnology Letters
Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube
Kobayashi, Tominari; Nakamura, Yusuke; Mikami, Satoshi; Masutani, Mamiko; Machida, Kodai; Imataka, Hiroaki
EMCV Rbz plasmid, which encodes full-length ...
... per recipient cell (t/r) using plasmid  pUC18 
European Food Research and Technology
Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria
Rizzi, Aurora; Brusetti, Lorenzo; Arioli, Stefania; Nielsen, Kaare M.; Tamagnini, Isabella; Tamburini, Alberto; Sorlini, Claudia; Daffonchio, Daniele
or a 1,873 bp fragment as donor DNA, and 3.5 × ...
... of a 60-base ssDNA substrate, a 60 bp dsDNA,  pUC18 
Nucleic Acids Research
The reverse gyrase helicase-like domain is a nucleotide-dependent switch that is attenuated by the topoisomerase domain
del Toro Duany, Yoandris; Jungblut, Stefan P.; Schmidt, Andreas S.; Klostermeier, Dagmar
plasmid DNA, and polyU-RNA at saturating ATP ...
... by PCR. They were inserted into vector  pUC18 
World Journal of Microbiology and Biotechnology
Enhanced 1,3-propanediol production in recombinant Klebsiella pneumoniae carrying the gene yqhD encoding 1,3-propanediol oxidoreductase isoenzyme
Zhu, Jian-Guo; Li, Shuang; Ji, Xiao-Jun; Huang, He; Hu, Nan
, yielding the recombinant expression vector ...
... LSA34 Given by Dr. Zhaolan Mo  pUC-gapA  pUC18 
Marine Biotechnology
Surface Display of Aeromonas hydrophila GAPDH in Attenuated Vibrio anguillarum to Develop a Noval Multivalent Vector Vaccine
Zhao, Yan; Liu, Qin; Wang, Xiuhua; Zhou, Lingyun; Wang, Qiyao; Zhang, Yuanxing
derivative; Plac-gapA fusion, Apr This study ...
... and cells carrying either the empty vector  pUC18 
Extremophiles
Cloning and identification of a novel NhaD-type Na+/H+ antiporter from metagenomic DNA of the halophilic bacteria in soil samples around Daban Salt Lake
Zhang, Hua; Wang, Zhenhui; Wang, Lei; Mu, Ren; Zou, Zhi; Yuan, Kun; Wang, Yuekun; Wu, Haiping; Jiang, Juquan; Yang, Lifu
, or the recombinant plasmids pUCNHD ( ...
... C09G4.1 pJW9 hyl-1 seq spanning Lag1p motif*  pUC18 
AGE
Genetic analysis of hyl-1, the C. elegans homolog of LAG1/LASS1
Tedesco, Patricia; Jiang, James; Wang, Jinqing; Jazwinski, S. Michal; Johnson, Thomas E.
None ...
... of supercoiled, EcoRI-linearized or nicked  pUC18 
Nucleic Acids Research
DNA protection by histone-like protein HU from the hyperthermophilic eubacterium Thermotoga maritima
Mukherjee, Anirban; Sokunbi, Abimbola O.; Grove, Anne
, prepared by incubation of ...
... and cells carrying either the empty vector  pUC18 
Extremophiles
Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis
Meng, Lin; Hong, Shan; Liu, Henan; Huang, Haipeng; Sun, Hao; Xu, Tong; Jiang, Juquan
or the recombinant plasmid pUC-mrp ( ...
... materials For binding experiments plasmid DNA (  pUC18 
Biotechnology Letters
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
Bonde, Johan; Bülow, Leif
), linear dsDNA (linearized ...
... transformed pEC into E. coli JM83 harboring  pUC18 
Biotechnology Letters
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Zhang, Huijing; Wu, Haizhen; Zhang, Huizhan
to be used as internal standard for evaluating ...
... A fragment of the DNA sequence of the  pUC18 
Measurement Techniques
Selection of a reference sequence for the determination of nucleotide sequences in DNA molecules
Golubev, S. S.; Kononogov, S. A.; Ravin, N. V.; Skryabin, K. G.
plasmid is proposed as a reference sequence for ...
... methods Reagents and biochemicals The plasmid  pUC18 
Radiation and Environmental Biophysics
Radioprotective effects produced by the condensation of plasmid DNA with avidin and biotinylated gold nanoparticles
Perry, Christopher C.; Urata, Sarah M.; Lee, Melissa; Aguilera, Joe A.; Milligan, Jamie R.
was grown on a liter scale in an E. coli host ...
... putative oriT RA3 (Figure 1B) was cloned into  pUC18 
BMC Microbiology
MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer
Godziszewska, Jolanta; Kulińska, Anna; Jagura-Burdzy, Grażyna
[23], it facilitated plasmid mobilization by ...
... was conducted as previously described 17 27 .  pUC18 
BMC Microbiology
Acid stress damage of DNA is prevented by Dps binding in Escherichia coli O157:H7
Jeong, Kwang; Hung, Kai; Baumler, David; Byrd, Jeffrey; Kaspar, Charles
plasmid DNA (300 ng) was mixed with varying ...
... DNA from plasmid  pUC18 
Radiation and Environmental Biophysics
Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation
Shiina, Takuya; Watanabe, Ritsuko; Shiraishi, Iyo; Suzuki, Masao; Sugaya, Yuki; Fujii, Kentaro; Yokoya, Akinari
was irradiated with low-LET (13 keV/μm) or ...